PowerPlex 16 Primer Pairs

PowerPlex 16 system 是由PROMEGA开发的STR检测试剂盒,用于亲子鉴定(paternity testing)、法医分析(forensic DNA analysis)、身份识别(human identity testing),以及品系鉴定(tissue culture strain identification)。包含全部13个CODIS STR LOCI(TPOX, D3S1358, FGA, CSF1PO, D5S818, D7S820, D8S1179, TH01,VWA, D13S317, D16S539, D18S51, D21S11), Penta D,Penta E,以及用于性别鉴定的Amelogenin,共计16个位点。

PowerPlex 16 System

引物信息如下

Locus Forward Reverse 5’ end
FGA GGCTGCAGGGCATAACATTA ATTCTATGACTTTGCGCTTCAGGA TMR /OH
TPOX GCACAGAACAGGCACTTAGG CGCTCAAACGTGAGGTTG OH /TMR
D8S1179 ATTGCAACTTATATGTATTTTTGTATTTCATG ACCAAATTGTGTTCATGAGTATAGTTTC OH /TMR
VWA GCCCTAGTGGATGATAAGAATAATCAGTATGTG GGACAGATGATAAATACATAGGATGGATGG OH /TMR
AMEL CCCTGGGCTCTGTAAAGAA ATCAGAGCTTAAACTGGGAAGCTG TMR /OH
PENTA E ATTACCAACATGAAAGGGTACCAATA TGGGTTATTAATTGAGAAAACTCCTTACAATTT OH /FL
D18S51 TTCTTGAGCCCAGAAGGTTA ATTCTACCAGCAACAACACAAATAAAC FL /OH
D21S11 ATATGTGAGTCAATTCCCCAAG TGTATTAGTCAATGTTCTCCAGAGAC OH /FL
TH01 GTGATTCCCATTGGCCTGTTC ATTCCTGTGGGCTGAAAAGCTC FL /OH
D3S1358 ACTGCAGTCCAATCTGGGT ATGAAATCAACAGAGGCTTGC OH /FL
PENTA D GAAGGTCGAAGCTGAAGTG ATTAGAATTCTTTAATCTGGACACAAG JOE /OH
CSF1PO CCGGAGGTAAAGGTGTCTTAAAGT ATTTCCTGTGTCAGACCCTGTT JOE /OH
D16S539 GGGGGTCTAAGAGCTTGTAAAAAG GTTTGTGTGTGCATCTGTAAGCATGTATC OH /JOE
D7S820 ATGTTGGTCAGGCTGACTATG GATTCCACATTTATCCTCATTGAC JOE /OH
D13S317 ATTACAGAAGTCTGGGATGTGGAGGA GGCAGCCCAAAAAGACAGA OH /JOE
D5S818 GGTGATTTTCCTCTTTGGTATCC AGCCACAGTTTACAACATTTGTATCT OH /JOE

参见文章:Published in Krenke, B. E., Tereba, A., Anderson, S. J., Buel, E., Culhane, S., Finis, C. J., Tomsey, C. S., Zachetti, J. M., Masibay, A., Rabbach, D. R., Amiott, E. A., and Sprecher, C. J. (2002) Validation of a 16-locus fluorescent multiplex system. J. Forensic Sci. 47(4): 773-785.

  • 本文作者:括囊无誉
  • 本文链接: BIO/PowerPlex 16 Primer Pairs/
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