PowerPlex 16 system 是由PROMEGA开发的STR检测试剂盒,用于亲子鉴定(paternity testing)、法医分析(forensic DNA analysis)、身份识别(human identity testing),以及品系鉴定(tissue culture strain identification)。包含全部13个CODIS STR LOCI(TPOX, D3S1358, FGA, CSF1PO, D5S818, D7S820, D8S1179, TH01,VWA, D13S317, D16S539, D18S51, D21S11), Penta D,Penta E,以及用于性别鉴定的Amelogenin,共计16个位点。
引物信息如下:
Locus | Forward | Reverse | 5’ end |
---|---|---|---|
FGA | GGCTGCAGGGCATAACATTA | ATTCTATGACTTTGCGCTTCAGGA | TMR /OH |
TPOX | GCACAGAACAGGCACTTAGG | CGCTCAAACGTGAGGTTG | OH /TMR |
D8S1179 | ATTGCAACTTATATGTATTTTTGTATTTCATG | ACCAAATTGTGTTCATGAGTATAGTTTC | OH /TMR |
VWA | GCCCTAGTGGATGATAAGAATAATCAGTATGTG | GGACAGATGATAAATACATAGGATGGATGG | OH /TMR |
AMEL | CCCTGGGCTCTGTAAAGAA | ATCAGAGCTTAAACTGGGAAGCTG | TMR /OH |
PENTA E | ATTACCAACATGAAAGGGTACCAATA | TGGGTTATTAATTGAGAAAACTCCTTACAATTT | OH /FL |
D18S51 | TTCTTGAGCCCAGAAGGTTA | ATTCTACCAGCAACAACACAAATAAAC | FL /OH |
D21S11 | ATATGTGAGTCAATTCCCCAAG | TGTATTAGTCAATGTTCTCCAGAGAC | OH /FL |
TH01 | GTGATTCCCATTGGCCTGTTC | ATTCCTGTGGGCTGAAAAGCTC | FL /OH |
D3S1358 | ACTGCAGTCCAATCTGGGT | ATGAAATCAACAGAGGCTTGC | OH /FL |
PENTA D | GAAGGTCGAAGCTGAAGTG | ATTAGAATTCTTTAATCTGGACACAAG | JOE /OH |
CSF1PO | CCGGAGGTAAAGGTGTCTTAAAGT | ATTTCCTGTGTCAGACCCTGTT | JOE /OH |
D16S539 | GGGGGTCTAAGAGCTTGTAAAAAG | GTTTGTGTGTGCATCTGTAAGCATGTATC | OH /JOE |
D7S820 | ATGTTGGTCAGGCTGACTATG | GATTCCACATTTATCCTCATTGAC | JOE /OH |
D13S317 | ATTACAGAAGTCTGGGATGTGGAGGA | GGCAGCCCAAAAAGACAGA | OH /JOE |
D5S818 | GGTGATTTTCCTCTTTGGTATCC | AGCCACAGTTTACAACATTTGTATCT | OH /JOE |
参见文章:Published in Krenke, B. E., Tereba, A., Anderson, S. J., Buel, E., Culhane, S., Finis, C. J., Tomsey, C. S., Zachetti, J. M., Masibay, A., Rabbach, D. R., Amiott, E. A., and Sprecher, C. J. (2002) Validation of a 16-locus fluorescent multiplex system. J. Forensic Sci. 47(4): 773-785.